Fig. 3: Scope and limitations of massive parallel protein tagging using ORFtag.
From: Proteome-scale tagging and functional screening in mammalian cells by ORFtag
a, Distribution of ORFtag integrations around TSSs of mouse protein-coding genes. b, Saturation curve displaying the relationship between the fraction of tagged proteins and the number of determined integration sites. c, Fraction of genes showing at least one integration in the combined background sample. d, Western blot against the FLAG tag assessing the tagging pattern in mES cell lysate before (−) and after (+) ORFtag transduction. This analysis was conducted on the first replicate of the PTGR screen. e, Ratio of protein-coding genes that were successfully tagged using ORFtag (ORFtag; pink) or were hits in any of the three screens (ORFtag hits; purple), compared to the distribution of ORF lengths across the whole mouse genome (genome; dashed line). Human ORFeome is shown for comparison (ORFeome; light gray). See Methods for further details. f, Ratio of protein-coding genes that were successfully tagged using ORFtag (ORFtag; pink) or were hits in any of the three screens (ORFtag hits; purple), compared to the distribution of ORF expression levels across the whole mouse genome (genome; dashed line).
