Extended Data Fig. 2: nalysis of motions for the pre-catalytic spliceosome.

a,d) Latent spaces of both half sets (half 1 and half 2) for the pre-catalytic spliceosome dataset EMPIAR-(10180) are coloured by the mean movement direction. Four different deformations are visualized as coloured (red, pink, yellow and blue) arrows from one point in latent space to another. b,e) The corresponding maps are shown in the same colours, with black arrows indicating the main deformations. c,f) For the blue and the pink deformations, 3D deformation fields are also shown as black arrows for the displacements of individual Gaussians. The latent spaces of the two half sets are organized in a similar way, with similar deformations along the shown directions. The observed motions are comparable to the ones obtained by e2gmm.