Extended Data Fig. 2: Optical layout of 4Pi-SIM.
From: Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM
Refer to the Methods section for a detailed description. The excitation laser (blue beam) emerges from the lower right, is reflected by the dichroic mirror (DM1), is then split by the non-polarizing beamsplitter (NPBS), and finally interferes at the sample plane. The emitted fluorescence (green beam) undergoes interference at the NPBS before being captured by the sCMOS camera (CAM1). The focus-lock laser (red beam) emerges from the upper left, is reflected by the dichroic mirror (DM2), is directed toward the objectives (OBJ1, 2), and is finally captured by a compact CMOS camera (CAM2). The excitation laser for I2M illumination (dark blue) emerges from the lower left and is reflected by a dichroic mirror (DM3) to the sample. AOTF: acousto-optic tunable filter; MMF: multimode fiber; SMF: single-mode fiber; PBS: polarization beam splitter; HWP: half-wave plate; SLM: spatial light modulator; HS-LCVR: high-speed liquid crystal variable retarder; DM: dichroic mirror; NPBS: non-polarizing beamsplitter; FTS: fine-tuning translation submodule; CTS: coarse-tuning translation submodule; PM: knife-edge right-angle prism protective silver mirror; QWP: quarter-wave plate; L1-L18: lenses; M1-M19: mirrors; CY1: cylindrical lens; and F1: filter.
