Extended Data Fig. 4: Rapid discriminative learning with true 3D objects in the binocular zone with non-grating and grating patterns.
From: Moculus: an immersive virtual reality system for mice incorporating stereo vision
a, Examples of a reward and a control zone from the viewpoint of mice. The reward zone was cued with a cube with a dotted pattern, while the control zone contained a cone decorated with stars. Both objects floated close to the middle bisector plane in the upper part of the binocular zone. Regardless of whether the 3D objects differed from those in Fig. 4d, the same reward protocol with two reward locations was applied: the second reward was provided at the end of the reward zone. The first reward was only given if mice started licking between the beginning of the zone and the location of the second reward. The second reward was only provided if the animal failed to trigger the first reward or showed no licking after reward delivery. b, Average licking rate before reward (mean ± SEM) timed to the entry into the control and rewarded zones (0 s) across multiple training days, broken into 10-minute intervals, comparing control (unrewarded, black) and reward (blue) zones. Only licking data before reward are included. Bar graphs showing average licking rates (mean ± SEM) in the control zone and in the reward zone before reward. Data from n = 6 mice, minimum 281 and 338 trials/day in reward and control zones, respectively (mean ± SD = 348.75 ± 49.02 and 395.75 ± 61.9, respectively). *: significant difference between the two zones, two-tailed t-test (pday1 = 7.39×10−37, pday2 = 8.22×10−30, pday3 = 1.30×10−33, pday4 = 7.94×10−54). c-d, The same protocol as in Fig. 4d, e. d, Left, licking rate (mean ± SEM) timed to the entry into the control and rewarded zones across multiple training days, broken into 10-minute intervals, comparing control (unrewarded, black) and reward (blue) zones. Data are from the measurements shown in Fig. 4d–g. Right, bar graphs showing average anticipatory licking rates (mean ± SEM) before entry into the control (black) and reward (blue) zones. Data from n = 5 mice, minimum 146 and 150 trials/day in control and reward zones, respectively (mean ± SD = 161.8 ± 19.79 and 168.8 ± 15.69, respectively). *: significant difference between the two zones (one-tailed t-test, pday1 = 0.032, pday2 = 0.0067, pday3 = 1.41×10−8, pday4 = 0.00038, pday5 = 1.80×10−8).
