Extended Data Fig. 9: Coding.
From: Moculus: an immersive virtual reality system for mice incorporating stereo vision
a-g, a three-neuron decoder provides orthogonal binary output for the assemblies coding the aversive and control zones. a, Average activity of the top 18 neurons from histogram in Fig. 5c in aversive (red) and control zones (gray). Note the gap in responses at about ─2.5 s, preceding velocity (blue) increase. b, Corresponding average responses. c, Left, summed activity of three neurons (=#25 + #28 + #59) from the 18 cells in aversive (red) and control (gray dotted) zones. Right, activity of the three cells integrated from -4 to -2.5 s defined a decoder with orthogonal outputs for the control and aversive zones, before running speed increase (mean ± SEM). Dark colors: high-ramp trials. Light colors: low-ramp trials. d, Similar to c but during high-ramp trials only. e, Correlation between running and decoder performance for all trials (Pearson’s r = 0.44) and for high-ramp trials (red dots, r = 0.70). f-g, Average response of hub cells mimics average ramping activity. f, Mean responses of the 10 aversive and 10 control hub cells (Fig. 5f) at zone ends. g, Average responses (mean ± SEM) of hub cells showing similarity to Fig. 3f. h-n, Weight factors analysis. h, Left, weight factors of the first TCA component (Fig. 6d). Right, after rescaling. i, Average responses (mean ± SEM) of neurons with high and low weight factors from h. j, Same as i but rescaled. Curves are presented as mean values ± SEM, k, Responses from i, with a new baseline showing a second peak component linked to reinforcement. l, Same neuronal responses as in i show overlapping ON response. m, The same as Fig. 5c, but neurons with high (red) and low (orange) weight factors indicated. Most high-weight cells align with hub cells. n, Panel from Fig. 3d showing neurons with high weight factors (triangle). o-p, Relationship between reinforcement and learning. o, Left, Responses of the 10 best (red) and 10 worst (black) learners (from Fig. 6b) at early learning normalized to the visual ON response, averaged (mean ± SEM) and subtracted (green); running speed is overlaid; definition of the first and second components is indicated. Right, correlation between velocity and response components. p, The same as the bottom part of Fig. 6b but for the control zone. The exact Pearson’s r values are shown in the figure and in the Source Data File.
