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Single-molecule techniques

Single-molecule DNA dynamics with graphene energy transfer

Nature Methods volume 22, pages 16–17 (2025)Cite this article

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Graphene energy transfer (GET) can be used to measure the distance between a graphene surface and a fluorescent dye. A new strategy for attaching DNA in a controlled orientation onto graphene now makes it possible to use GET to track the dynamics of DNA and protein–DNA interactions with base-pair resolution.

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Fig. 1: Graphene energy transfer (GET) with vertically oriented DNA enables single-molecule measurements of DNA structure and protein–DNA interactions.

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Acknowledgements

I thank Ryan Fantasia, Amanda Wacker and Delisa Ramos for insightful comments and discussions. I am grateful for support from the Arnold and Mabel Beckman Foundation and from the W.M. Keck Foundation.

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  1. Salk Institute for Biological Studies, La Jolla, CA, USA

    Pallav Kosuri

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  1. Pallav Kosuri
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Correspondence to Pallav Kosuri.

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The author declares no competing interests.

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Kosuri, P. Single-molecule DNA dynamics with graphene energy transfer. Nat Methods 22, 16–17 (2025). https://doi.org/10.1038/s41592-024-02560-8

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