Extended Data Fig. 9: ACE cluster-wise statistical analysis for unraveling neuronal ensembles controlling walking. | Nature Methods

Extended Data Fig. 9: ACE cluster-wise statistical analysis for unraveling neuronal ensembles controlling walking.

From: A deep learning pipeline for three-dimensional brain-wide mapping of local neuronal ensembles in teravoxel light-sheet microscopy

Extended Data Fig. 9

a. Voxelized and warped segmentation maps, obtained by integrating ACE’s segmentation module and MIRACL registration algorithms, were averaged per group. The average of the homecage (non-walking) group was subtracted from the walking group to obtain a density heatmap, demonstrating neuronal hotspots differentially activated in the walking group. b. Statistical whole-brain p-value map obtained by ACE cluster-wise analysis with a group-wise two-way ANOVA, demonstrating several sub-regional and laminar neuronal clusters differentially activated during walking.

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