Extended Data Fig. 3: OLS enables reproducible and robust SMT measurements. | Nature Methods

Extended Data Fig. 3: OLS enables reproducible and robust SMT measurements.

From: Oblique line scan illumination enables expansive, accurate and sensitive single-protein measurements in solution and in living cells

Extended Data Fig. 3: OLS enables reproducible and robust SMT measurements.

(a) EC50 values calculated from each averaged dose-response curve per plate per microscope, solid lines represent the median EC50 per microscope measure from 6-7 384-well plates. (b) Violin plot of the signal to noise ratio (SNR) per microscope, heavy dashed line represents median value and light dashed lines represent 1st and 3rd quartiles. Data is sampled from 6 FOVs for each of 308 wells from 6-7 384-well plates. (c) Violin plot of the SNR as a function of FOV position within an acquired well, heavy dashed line represents median value and light dashed lines represent 1st and 3rd quartiles Data is sampled from 308 wells from 6-7 384-well plates (d) 20-point dose-response curve for Halo-KEAP1 U2OS sampled at full OLS FOV (purple) versus a cropped FOV of 768 ×768 pixels (black) representative of a HILO-sized FOV. Error bars denote standard deviation between FOVs.

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