Extended Data Fig. 7: Signal-to-noise in vivo analysis of the PTEN sensor.
From: Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain
a Representative images of fluorescent lifetime summed over different frames numbers, for G-PTEN in single L2/3 cells in vivo. Representative images represent experiments repeated independently at least 3 times. Scale bar; 20 μm. b Standard deviation of signal-to-noise of G-PTEN in vivo, as a function of the amount of photons from each cell. Each point represents individual cells for frame-to-frame (left) or individual experiments over one field of view for cell-to-cell (right). The binding fraction’s theoretical change caused by shot-noise is represented by the black curve (see methods for equation).
