Extended Data Fig. 9: Dark sectioning assists in long-term observation of live cells. | Nature Methods

Extended Data Fig. 9: Dark sectioning assists in long-term observation of live cells.

From: Dark-based optical sectioning assists background removal in fluorescence microscopy

Extended Data Fig. 9

(a) Comparisons between WF and DarkWF of ER tubes in live COS-7 cells with a plasmid expressing GFP-tagged KDEL protein. (b) Comparisons between WF and DarkWF of tubulin (labeled by SiR Tubulin Kit, Cytoskeleton, CY-SC002) and lysosomal (labeled by LysoView™ 488, Biotium, 70067) in live COS-7 cells. (c) Comparisons between WF, DarkWF, and SIM, DarkSIM of mitochondria labeled by PKmito RED (Cytoskeleton, CY-SC052). Scale bar: 4 μm. Lateral scale bar: 4 μm.

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