Extended Data Fig. 2: Transsynaptic labeling in culture with ATLAS.
From: ATLAS: a rationally designed anterograde transsynaptic tracer
a. Presynaptic neuron labeled with At. b. Postsynaptic neurons labeled with GFP in same experiment as in a. c. Merge of a. and b. showing presynaptic neuron (At) labeled in magenta and postsynaptic neurons (GFP) labeled in green. d. Closeup of boxed region d from panel c. showing adjacent/overlapping presynaptic termini (magenta) and dendrites or spines (green) indicated by arrowhead, consistent with synaptic contacts between the presynaptic neuron in a. and postsynaptic neuron 1 in c. e. Closeup of boxed region e from panel c. showing adjacent/overlapping presynaptic termini (magenta) and dendrites or spines (green) indicated by arrowhead, consistent with synaptic contacts between the presynaptic neuron in a. and postsynaptic neuron 2 in c. f. Closeup of boxed region f from panel c. showing adjacent/overlapping presynaptic termini (magenta) and dendrites or spines (green) indicated by arrowhead consistent with synaptic contacts between the presynaptic neuron in a. and postsynaptic neuron 3 in c. g. Closeup of boxed region g from panel c. showing adjacent/overlapping presynaptic termini (magenta) and dendrites or spines (green) indicated by arrowhead. Scale bars 100 μm in c, 10 μm in d-g.
