Fig. 2: RDM for miniature microscopy. | Nature Methods

Fig. 2: RDM for miniature microscopy.

From: Ring deconvolution microscopy: exploiting symmetry for efficient spatially varying aberration correction

Fig. 2

After calibrating the Miniscope with a single image of fluorescent beads (Fig. 1b), we show results from several deblurring methods for comparison: standard deconvolution (using a single measured PSF), a U-Net trained on our spatially varying blur dataset, ring deconvolution and DeepRD. Deconvolution assumes space-invariance while the remaining methods are designed to handle spatially varying aberrations. In the first row, ring deconvolution and DeepRD clearly resolve resolution target elements near the edges of the FoV, which are not as well resolved by the two other methods. Zoom-ins show RDM resolves up to element 6 of group 9 (blue inset) and element 5 of group 8 (green inset). Similar results along with corresponding insets are shown for the other samples.

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