Fig. 3: RDM for high-NA, multicolor fluorescence microscopy. | Nature Methods

Fig. 3: RDM for high-NA, multicolor fluorescence microscopy.

From: Ring deconvolution microscopy: exploiting symmetry for efficient spatially varying aberration correction

Fig. 3

a,b, Fluorescent beads imaged with a ×100 1.4 NA objective (a) and corresponding Seidel-fitted PSFs demonstrate spatially varying nature of the system (b). c, Two representative examples of BPAE cells processed by standard deconvolution and RDM. Deconvolution and RDM perform similarly in the center but RDM is better in the corner, revealing submicron features in the actin (orange arrow). RDM similarly resolves actin filaments and mitochondria where deconvolution does not. A total of six such samples were prepared and imaged with similar results.

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