Extended Data Fig. 1: RDM for space-invariant systems.

Our Seidel and blind deconvolution algorithms compared with standard deconvolution on USAF test target and live tardigrade images from the Miniscope. The field-of-view is cropped to ensure space-invariance. Our methods outperform standard deconvolution by using a synthetic PSF, which prevents artifacts and loss of resolution from noise-based artifacts. Our blind deconvolution method usually correctly estimates the spherical Seidel coefficient well; however, for certain images, the blind method can overestimate the coefficient, leading to over sharpening of the image, as in the USAF resolution chart.