Fig. 3: Experimental comparisons of SeReNet and other SOTA methods in diverse living organisms.
a, Orthogonal maximum intensity projections (MIPs) showing the process of migrasome formation in a zebrafish embryo, obtained by SeReNet and iterative tomography. b, Normalized intensity profiles along the two lines marked in a. c, Center view and MIP obtained by SeReNet of membrane-labeled D. discoideum at t = 192 s, with white arrows indicating produced EVs and yellow arrows pointing to motion artifacts. The amoebas were cultured in a dish, as shown in the cartoon. d, Tracking of D. discoideum based on SeReNet results. The tracking traces were obtained through Imaris 9.0.1 software, with an overall tracking time of 1,260 s. A total of 49 cells were tracked with temporal-coding trajectory. The colors reflect different time points. e, Enlarged MIPs showing EV generation from D. discoideum at different stamps, comparing different methods. Profiles across an EV are compared, and yellow arrows indicate motion artifacts. f, Dual-directional MIPs and enlarged regions of an entire NeuroPAL worm (strain OH16230) obtained by SeReNet and iterative tomography. g, Orthogonal MIPs of neuron-labeled worm midbody by SeReNet, with enlarged regions showing comparisons between different methods. The identity is marked on the side of each neuron. h, Normalized intensity profiles along the marked dashed lines in g. i, Temporal traces (ΔF/F0) of GCaMP6s transients in four neurons, extracted from results of different methods. All networks were trained on the synthetic bubtub dataset, and the SeReNet here was the axially improved version. Scale bars, 10 μm (a,c–e, enlarged views in f,g), 50 μm (f,g, original views).
