Fig. 4: High-fidelity long-term imaging by SeReNet in mice with liver injury.
a, Illustrations of the LIRI model. Mice were anesthetized and subjected to hepatic ischemia and reperfusion. After 24 h, liver regeneration was initiated. b, Boxplot showing the neutrophil counts in mouse livers without and with LIRI. n = 4 regions. P = 5.89 × 10−3. c, Orthogonal MIPs by SeReNet showing neutrophils (Ly6G, green) and KCs (F4/80, magenta) in the vessels (WGA, blue) of living mouse livers following LIRI, with enlarged MIPs showing the interactions between neutrophils and KCs. Arrows indicate the image blur and artifacts. d, Retraction fiber length of the KC over 15 min, showing the elongation process. e, MIPs showing that a KC stretched out a retraction fiber to touch another KC. The lengths of the two retraction fibers over 75 min are plotted. f, MIPs showing that neutrophils generated a long retraction fiber and produced a migrasome that was delivered into a KC. The retraction fiber length over 60 min is plotted. g, Illustrations of the AILF model. Mice were given an i.p. injection of APAP (600 mg kg–1) for 16 h to induce a proinflammatory phenotype. h, Boxplot showing the count of CD63+ ECs in mouse livers without and with AILF. n = 4 regions. P = 1.17×10−2. i, MIPs obtained by SeReNet of monocytes (Ly6C, green) and CD63+ ECs (CD63, magenta) in the vessels (WGA, blue) of livers of living mice with AILF. Arrows indicate the image blur and artifacts. j,k, Enlarged MIPs of two regions demonstrate the proximity process (j) and adhesion process (k) between monocytes and CD63+ ECs. The color-coded trajectories of two monocytes are overlaid. l, Centroidal distances between the monocytes and CD63+ ECs over time. In boxplots: center line, median; box limits, lower and upper quartiles; whiskers, 0th–100th percentiles. Asterisks represent significance levels tested with two-sided paired t-test. Significance at P < 0.05. *P < 0.05; **P < 1 × 10−2. SeReNet was trained on the synthetic bubtub dataset. Scale bars, 10 μm (c,e,f,i–k).
