Extended Data Fig. 6: Cluster-based pseudobulking strategy and transcription factor connectivity of the macrophage polarization eGRN. | Nature Methods

Extended Data Fig. 6: Cluster-based pseudobulking strategy and transcription factor connectivity of the macrophage polarization eGRN.

From: Single-cell ultra-high-throughput multiplexed chromatin and RNA profiling reveals gene regulatory dynamics

Extended Data Fig. 6: Cluster-based pseudobulking strategy and transcription factor connectivity of the macrophage polarization eGRN.

a, Weighted nearest neighbor (WNN) UMAP projection of integrated SUM-seq data from macrophage polarization, colored by the cell clusters used for pseudobulking and subsequently inferring an eGRN. Only clusters with more than 25 cells were included to make the eGRN. b, Degree plot of number of target genes linked to each transcription factor (TF) in the eGRN. Only TFs with at least 4 connected genes are shown.

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