Extended Data Fig. 7: Dynamics of STAT1, STAT2, and IRF9 motif and regulon activity in M1 polarization. | Nature Methods

Extended Data Fig. 7: Dynamics of STAT1, STAT2, and IRF9 motif and regulon activity in M1 polarization.

From: Single-cell ultra-high-throughput multiplexed chromatin and RNA profiling reveals gene regulatory dynamics

Extended Data Fig. 7: Dynamics of STAT1, STAT2, and IRF9 motif and regulon activity in M1 polarization.

a, Heatmaps showing TF motif activity (rank normalized chromVAR z-score), regulon activity (rank normalized AUC score), and the similarity between the two, for M1 polarization-associated TFs found as TFs in the eGRN, across M0 and M1 cells sorted by the M1 sustained factor. b, Scatter plots of the correlation between the STAT2 regulon activity (AUC score) and TF motif activity (chromVAR Z-score) and c, IRF9 regulon activity and motif activity. Points represent cells and are coloured by their experimental time point. d, Venn diagram of STAT1, STAT2 and IRF9 regulon genes. e, Scatter plots of the correlation between STAT1.1 and IRF9 motif activities (top) and STAT1 and IRF9 regulon activities (bottom). Points represent cells and are coloured by their experimental time point. f, Scatter plots of the correlation between STAT1.1 and STAT2 motif activities (top) and STAT1 and STAT2 regulon activities (bottom). Points represent cells and are coloured by their experimental time point. g, STAT1 T699 and Y701 phosphopeptide intensities upon IFN-y/LPS stimulation of THP1 derived macrophages. h, IRF9 S131 and S253 phosphopeptide intensities upon IFN-y/LPS stimulation of THP1 derived macrophages.

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