Extended Data Fig. 3: Visualizing multi-cellular structures, cell shape, and motility in native tissue.
From: Highly multiplexed 3D profiling of cell states and immune niches in human tumors
a, Surface rendering of 5-μm virtual section of the blood vessel from Fig. 2a, viewed from above, showing B cell undergoing trans-endothelial migration (diapedesis). Scale bar 10 μm. b, Surface rendering of a different B cell (yellow) elsewhere in dermis of melanoma in-situ migrating through vessel wall (green) Scale bar 10 μm. c, T cell inside vessel, shown as a surface rendering (left) and as volumetric rendering (right). d, B cells in the VGP dermis with a characteristic round morphology. e, Sphericity (left) and volume (right) of B cells in the entire MIS (n = 15) and VGP (n = 352) regions from 1 biological replicate from 1 specimen (Dataset 1 - LSP13626). Data is represented as mean +/-SD.
