Extended Data Fig. 7: Isolation of 1.4 nm-HAN-488 Labeled ribosomes from HEK 293 T L29-Halo cells. | Nature Methods

Extended Data Fig. 7: Isolation of 1.4 nm-HAN-488 Labeled ribosomes from HEK 293 T L29-Halo cells.

From: ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms

Extended Data Fig. 7: Isolation of 1.4 nm-HAN-488 Labeled ribosomes from HEK 293 T L29-Halo cells.The alt text for this image may have been generated using AI.

(a) UV Absorbance profile following sucrose gradient of ribosomal particles isolated from approximately 3.2 ×107 HEK 293 T L29-Halo cells treated with SLO and delivery of 4 uM 1.4 nm-HAN-488 probes. Experiment was performed once. (b) Central slice of a representative reconstructed tomogram from 1.4 nm HAN-488 labeled ribosomes, right inset to show an individual ribosome, from 167 tomograms. (c) Orthoslices from unlabeled ribosomal class, and the nanogold-labeled ribosomal class with classification distribution, from 26,413 picked particles. Scale bars: b, 50 nm (left), 25 nm (right).

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