Extended Data Fig. 3: Nanogold enhancement is specific to HEK 293 T RPL29-Halo. | Nature Methods

Extended Data Fig. 3: Nanogold enhancement is specific to HEK 293 T RPL29-Halo.

From: ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms

Extended Data Fig. 3: Nanogold enhancement is specific to HEK 293 T RPL29-Halo.

(a) Transmission electron microscopy (TEM) images of HEK 293 T cells that were enhanced for 10 min. Left: WT cells without Halo-nanogold, middle: WT cells treated with 3 uM 1.4 nm-HAN-488; right: RPL29-Halo cells treated with 3 uM 1.4 nm-HAN-488. The scale is 2 um. (b) Immunofluorescence images of HEK 293 T cells with DAPI and phalloidin staining as a maker for the cell edge. (c) Quantification of cell volume from the cells shown in A. N = 10 cells. Mean =5283, error bars show SD, SD = 1.85. (d) Quantification of cell volume in TEM images. N = 9. Mean =10.05, error bars show SD, SD = 2.1. (e) Quantification of ribosomal copy number in HEK 293 T RPL29-Halo cells, Mean= 1.4×106, SD = 4.8×105, N = 6 independent experiments.

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