Fig. 2: DBiTplus performance and spatial multi-omic analysis of FFPE mouse embryo.
a, Brightfield images of an E11 FFPE mouse embryo section before (top) and after (bottom) the DBiTplus workflow, showing that tissue integrity and morphology are preserved following cDNA retrieval (n = 2). b, Correlation analysis between two FFPE E11 mouse embryo section samples comparing the standard DBiT-seq and the DBiTplus workflows from a two-sided Pearson correlation test (Pearson correlation = 0.99 and P value < 2.2 × 10−16). c, Venn diagram showing overlap of genes sequenced between the standard DBiT workflow and the DBiTplus workflow. d, Distribution of detected genes and UMIs per spatial spot. Blue and red dashed lines represent the average gene and UMI counts, respectively. e, Top, UMAP clustering of spatial transcriptomic data identified ten distinct transcriptomic clusters from the E11 mouse embryo. Bottom, mxIF staining performed on the same tissue section. SMA, smooth muscle actin. f, Comparison of the spatial gene expression (DBiTplus) and protein expression (CODEX) for selected markers reveals concordant spatial localization across both modalities. g, CODEX-informed spot deconvolution of DBiTplus data. Cells were annotated by label transfer from the MOCA dataset using MaxFuse.
