Extended Data Fig. 3: Spatial profiling of gene expression in FFPE mouse embryo using DBiTplus. | Nature Methods

Extended Data Fig. 3: Spatial profiling of gene expression in FFPE mouse embryo using DBiTplus.

From: Integration of imaging-based and sequencing-based spatial omics mapping on the same tissue section via DBiTplus

Extended Data Fig. 3: Spatial profiling of gene expression in FFPE mouse embryo using DBiTplus.

a UMAP showing 10 distinct clusters of DBiTplus spots/spots. b Spatial distribution of the 10 clusters of DBiTplus spots. c Heat map showing the top 5 differentially expressed genes for each spatial cluster. d Spatial distribution of clusters 0, 1, and 2 alongside their principal defining genes. In-situ Hybridization (ISH) staining image from the Allen Mouse Brain Atlas (Developing Mouse Brain - Age: E13.5, Theiler Stage: TS21) and gene expression from STOmics Mouse Organogenesis Spatiotemporal Transcriptomic Atlas (MOSTA) dataset (E13.5_E1S1.MOSTA.h5ad). Scale bar 1.5 mm for Allen Mouse Brain Atlas images.

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