Fig. 3: CaBLAM provides high-contrast reporting of stimulus-evoked neural activity in cultured neurons. | Nature Methods

Fig. 3: CaBLAM provides high-contrast reporting of stimulus-evoked neural activity in cultured neurons.

From: CaBLAM: a high-contrast bioluminescent Ca2+ indicator derived from an engineered Oplophorus gracilirostris luciferase

Fig. 3

a, Bioluminescence ΔL/L time-locked traces of CaBLAM Ca2+ responses to 1, 5 and 40 pulses of 1-ms field stimulations at 83 Hz (red dashed lines indicate field stimulation window). b, Same as a, but fluorescence ΔF/F time-locked traces for GCaMP8s. For a and b, data are shown as mean ± s.e.m. c, Peak stimulus-evoked ΔF/F or ΔL/L across neurons across increasing field stimulations. d, Cumulative distribution of time to peak ΔF/F or ΔL/L responses. e, Time to peak ΔF/F or ΔL/L across neurons in response to increasing field stimulations. f, Peak SNR of ΔF/F or ΔL/L responses across neurons at increasing stimulation intensities. For CaBLAM, data were pooled from 7 independent imaging sessions, with the following total number of neurons (n) analyzed at the corresponding stimulation numbers (1, 5 and 40): n = 61, 58 and 83. For GCaMP8s, data were pooled from 3 independent imaging sessions, with n = 15, 37 and 38 neurons analyzed at the same respective stimulation numbers. All boxplots show the median, 25th and 75th percentiles (box edges), whiskers extending to the most extreme data points and individual outliers plotted separately.

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