Extended Data Fig. 1: Characterizing furimazine dose-dependent performance of CaBLAM in cultured neurons. | Nature Methods

Extended Data Fig. 1: Characterizing furimazine dose-dependent performance of CaBLAM in cultured neurons.

From: CaBLAM: a high-contrast bioluminescent Ca2+ indicator derived from an engineered Oplophorus gracilirostris luciferase

Extended Data Fig. 1

a, Overlaid bioluminescence ΔL/L time-locked traces of CaBLAM Ca2+ responses to 1, 2, 10, 40, 160 pulses of 1 ms field stimulations at 83 Hz, at 4.6 μM or 9.2 μM Fz concentration. Red dashed lines indicate field stimulation window. Data are shown as mean ± s.e.m. b, Peak stimulus ΔL/L for CaBLAM across neurons elicited across increasing field stimulations. c, Cumulative distribution of time to ΔL/L responses between (includes statistical analysis). d, Proportion of neurons responding to 1 (left) and 40 (right) electrical field stimulations. All boxplots show the median, 25th and 75th percentiles (box edges), whiskers extending to the most extreme data points, and individual outliers plotted separately.

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