Extended Data Fig. 3: Bioluminescence time course with a single direct cortical application of FFz in a pan-neuronal CaBLAM mouse. | Nature Methods

Extended Data Fig. 3: Bioluminescence time course with a single direct cortical application of FFz in a pan-neuronal CaBLAM mouse.

From: CaBLAM: a high-contrast bioluminescent Ca2+ indicator derived from an engineered Oplophorus gracilirostris luciferase

Extended Data Fig. 3

All data is expressed in units of ΔL/L0, where baseline (L0) consisted of a 1 min recording prior to FFz administration (first orange marker in b). The imaging was then paused for ~2 min in order to administer the FFz, after which recording recommenced (time 0). a, top row, average projection images from each hour (600 images/h); bottom row, first single frames from each hour after FFz administration. b, Bioluminescence from a circular ROI (black circle in a, top row, second from left) binned at 10-min intervals. Error bars represent ± 1 SD across all pixels in the ROI.

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