Extended Data Fig. 4: Tactile responses over time in an anaesthetized pan-neuronal CaBLAM mouse with a single direct cortical application of FFz.

a, Average CaBLAM tactile response from ROI marked in Extended Data Fig. 3a, binned at 30-min intervals after the administration of FFz. b, Box plot of the single-trial peak ΔL/L0 binned at 30-min intervals. Orange lines indicate the median, boxes enclose the middle 2 quartiles of the data, whiskers extend 1.5 IQRs above and below. Gray dots are single data points. A Kruskal-Wallace test indicated a significant interaction of peak ΔL/L0 single-trial responses and time bin (10 30-min bins; P = 4.52 ×10−6; χ2 = 41.24, df = 9). P-values from follow-up comparisons (Tukey’s HSD) are indicated above horizontal black bars. c, box plot of SNR, same conventions as in b. SNR was not significantly different across time bins (Kruskal-Wallace test, P = 0.82, χ2 = 5.11, df = 9).