Table 1 Properties of selected intensiometric genetically encoded Ca²⁺ indicators

GECI name	Type	K_D (nM)^a	Hill coefficient^b	Contrast^c	Ref.
GCaMP8s	FP	46	2.2	50	⁸
GCaMP8f	FP	334	2.1	79	⁸
NEMOf	FP	528	3.2	246	⁴
NEMOc	FP	557	3.8	422	⁴
CaMBI_110	Luciferase	59 ± 6 (110)	0.97 ± 0.06 (2.5)	4.8 ± 0.1 (8)	¹⁸
GeNL(Ca²⁺)_480	Luciferase	457 ± 20 (480)	2.28 ± 0.21 (3)	3.6 ± 0.3 (5)	¹⁷
CaBLAM	Luciferase	439 ± 14	2.62 ± 0.18	83 ± 9	This work
CaBLAM_294W	Luciferase	3067 ± 95	2.24 ± 0.19	624 ± 39	This work
CaBLAM_332W	Luciferase	281 ± 9	2.26 ± 0.14	68 ± 5	This work

^aBinding constant for Ca²⁺ for FP-based GECIs as reported in the original publications; binding constant for Ca²⁺ as measured in vitro for CaMBI, GeNL(Ca²⁺)_480, CaBLAM and CaBLAM variants and (in parentheses) as reported in the original publications.
^bHill coefficient (cooperativity) for FP-based GECIs as reported in the original publications; Hill coefficient for luciferase-based GECIs calculated from sigmoidal curve fit to Ca²⁺ titration series and (in parentheses) as reported in the original publications.
^cContrast between Ca²⁺-free and Ca²⁺-saturated indicator in vitro for FP-based GECIs as reported in the original publications; contrast for luciferase-based GECIs measured in this work and (in parentheses) as reported in the original publications. Error margins represent 95% confidence interval (CI) for K_D and Hill coefficient and s.e.m. for contrast, as measured in this work.

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