Extended Data Fig. 5: In vivo benchmarking of nLightR2 during tail-lifting.
From: Next-generation multicolor indicators for in vivo imaging of norepinephrine
a, Experimental design: Fiber photometry to record norepinephrine (NE) release in the lateral hypothalamus (LH) using either nLightR or nLightR2. b, Behavioral paradigm. Tail Lift (TL): 1 m, 5 times. c, ∆F/F0 during TL. Solid lines represent the mean (nLightR, n = 5 mice; nLightR2, n = 6 mice) +/- SEM (shading). Min to peak ∆F/F0 before, during and after TL. (p = 0.008, Two-Way-ANOVA: Variant x Event interaction). d, Peri-event (140 s) heat maps showing ∆F/F0 for both variants. Rows represent individual trials (nLightR, 25 trials; nLightR2, 30 trials). Dashed line indicated TL duration. e, Area under the curve (AUC) of ∆F/F0 during the TL duration (p = 5.048e-007, two-tailed t-test).
