Extended Data Fig. 7: Spectral properties of nLightR2 in vivo.
From: Next-generation multicolor indicators for in vivo imaging of norepinephrine
a. Images showing nLightR2 fluorescence in the CA1 hippocampal layer of a head-fixed mouse for the red channel (top row) and the green channel (bottom row). Images are average fluorescence intensity (F) projections of recorded t-series acquired at the indicated excitation wavelengths. Scale bar, 50 µm. b. Mean, 25th, 50th, 75th, and 90th percentile values of the single-frame fluorescence intensity distribution are plotted as a function of the excitation wavelength for both the red (top row) and green (bottom row) channels. The color code indicates animal identity. Black lines and grey shaded areas represent the average across animals ± s.d. Values are computed over 3600 recorded frames.
