Extended Data Fig. 9: Two-photon hippocampal imaging of nLightR2-ctr during virtual spatial navigation.
From: Next-generation multicolor indicators for in vivo imaging of norepinephrine
a. Two-photon imaging was performed in head-fixed awake animals navigating in a virtual corridor. b. Longitudinal two-photon imaging was done while mice navigated for two consecutive days a familiar virtual corridor where they received water rewards at 85 cm from the start. c. Schematics of the hippocampus indicating the transduction of nLightR2-ctr in the CA1 pyramidal layer (pink, with gray contour). d. Representative average temporal projection of a FOV showing nLightR2-ctr fluorescence in the two consecutive experimental days. Projections are scaled to their maximum intensity value. Scale bars 50 μm. e. Event-triggered averages of nLightR2-ctr fluorescence over the whole FOV upon running (gold) or crossing the reward position (teal) in the two consecutive days. f-g, Same as in (e) for speed (f) and normalized (norm.) lick rate (g). h, The average nLightR2-ctr fluorescence over the whole FOV upon running (gold) or crossing the reward position (teal) are shown as a function of running speed over the two recording days. i, Average nLightR2-ctr fluorescence over the whole FOV upon crossing the reward position shown as a function of normalized lick rate in the two consecutive recording days. In (h-i) n.s., not significant two-sided rank-sum test; H0, slope of the linear model equals to 0. In (h), P values are: day 1, P(run) = 5.13 × 10−1, P(reward) = 1.00; day 2, P(run) = 5.13 × 10−1, P(reward) = 1.00. In (i), P values are: day 1, P(reward) = 2.48 × 10−1; day 2, P(reward) = 2.48 × 10−1. In panels (e-i), thick lines and shaded areas represent mean ± s.d. In (e-i), running epochs contain data from 3 animals, while reward crossing epochs have data from 4 animals.
