Extended Data Fig. 8: Optotagging with Neuropixels Opto probes preserves spike waveforms.

a. Schematic of reagents used in an example experiment. b. Virtual slice through light sheet volume of the brains used for this experiment, showing the location of the probe track and the regions of blue- or red-shifted opsin expression. c. Summary of unit locations within 100 µm of an emitter. Blue-tagged units appear as blue dots; there were no red-tagged units in this experiment. Large dots indicate units that pass quality metric thresholds for the complete session (ISI violations ratio < 0.5, amplitude cutoff < 0.1, presence ratio > 0.8). Small dots indicate units that pass the ISI violations ratio threshold only for the pre-stimulus baseline interval. Numbers indicate location of example units shown in panel d. d. Example waveforms extracted for each of four units. Each plot shows 50 randomly selected waveforms and their median (black), measured during the 10 ms light pulses (‘Evoked’) and in the interval between trials, when no light stimulus was present (‘Baseline’).