Extended Data Fig. 10: Additional examples of optotagged units.

a. Schematic of reagents used in five different experiments. b. Summary of unit locations within 100 μm of an emitter, showing red-tagged units (red dots), blue-tagged units (blue dots), and untagged units (black). Large dots indicate units that pass quality metric thresholds for the complete session, while small dots indicate units that pass the ISI violations ratio threshold only for the pre-stimulus baseline interval. Numbers indicate location of example units shown in panel c. c. Stacked raster plots aligned to 20 Hz laser pulses delivered from 14 emitters, for four example units from each session. Colored vertical bars indicate the time of each laser pulse, as well as the opsin (blue- or red-shifted) that this unit expresses. If units responded to both blue and red laser presentations, they were considered to be expressing a red-shifted opsin. d. Virtual slices through light sheet volumes of the brains used for the same optotagging experiments shown in panels a-c, showing the location of the probe track and the regions of blue- or red-shifted opsin expression. Image colors are based on the colors shown in the schematics in panel a. White arrows indicate the probe track in cases where it is faint. In the bottom row, the expanded region shows examples of fluorescent cell bodies that are difficult to see in the zoom-out view.