Extended Data Fig. 1: Annotation‑guided long‑read assembly performance across three modalities. | Nature Methods

Extended Data Fig. 1: Annotation‑guided long‑read assembly performance across three modalities.

From: StringTie3 improves total RNA-seq assembly by resolving nascent and mature transcripts

Extended Data Fig. 1: Annotation‑guided long‑read assembly performance across three modalities.The alternative text for this image may have been generated using AI.

Per‑replicate scatter plots of precision (%) (x‑axis) versus the number of reference transcripts correctly reconstructed by each assembler (y‑axis; ×103) for assemblies run in annotation‑guided mode against the CHESS 3.0.1 reference. Rows show (top to bottom): neuron‑differentiation ONT cDNA (days 0/3/5; three biological replicates each), LRGASP ONT dRNA (H1‑mix and WTC11; three replicates each), and LRGASP PacBio cDNA (H1‑mix and WTC11; three replicates each). Colors denote methods (StringTie2, StringTie3, StringTie3 nascent mode, IsoQuant, Bambu); shapes indicate replicate identity (see panel legends). “X” marks the per‑method mean across replicates.

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