Supplementary Figure 2: Oxytocin receptor expression in the parabrachial nucleus
From: Oxytocin-receptor-expressing neurons in the parabrachial nucleus regulate fluid intake
(a) Coronal sections 90 µm apart from Oxtr Cre/+ ::Ai14 mouse demonstrating oxytocin receptor (Oxtr) expression in the parabrachial nucleus (PBN) from bregma −5.1 to −5.5; scale bar, 500 µm. (b) Selection of brain images demonstrating robust Oxtr expression; AD, anterodorsal thalamic nucleus; CeA, central nucleus of amygdala; EPd, dorsal endopiriform nucleus; DMV, dorsal motor nucleus of the vagus; DR, dorsal raphé nucleus; GP, globus pallidus; XII, hypoglossal nucleus; MD, mediodorsal thalamic nucleus; NAc, nucleus accumbens; PBN, parabrachial nucleus; PVT, paraventricular thalamic nucleus; pVH, periventricular nucleus of the hypothalamus; SFO, subfornical organ; V, trigeminal motor nucleus; VMH, ventromedial hypothalamic nucleus; scale bar, 500 µm (n = 3). (c) Representative RNAscope® image of PBN demonstrating coexpression of Oxtr mRNA in 80 ± 3% Oxtr:TdTomato-expressing neurons (n = 3). Scale bar, 100 μm; scp, superior cerebellar peduncle. (d) Oxtr agonist, TGOT, increased spiking frequency in OxtrPBN neurons by 3.7 ± 0.55-fold, which was inhibited by Oxtr antagonist, atosiban to 1.6 ± 0.14 fold (n = 4/4 OxtrPBN neurons). Data were normalized to spiking frequency prior to TGOT application
