Supplementary Figure 6: OxtrPBN neurons in mid and rostral PBN show no significant difference in Fos expression
From: Oxytocin-receptor-expressing neurons in the parabrachial nucleus regulate fluid intake
a,c,e, Quantification of mid OxtrPBN co-expression of Fos and Oxtr in (a) salt depletion (n = 4 salt returned, 4 salt depleted, 3 control; 1-way ANOVA; Fos/Oxtr: interaction F(2,7) = 2.727; p = 0.1331; Oxtr/Fos: interaction F(2,7) = 0.3097; p = 0.7432), (c) fluid deprivation (n = 4 fluid returned, 3 fluid deprived; unpaired 2-tailed Student’s t-test; Fos/Oxtr: t(5) = 1.335; p = 0.2394; Oxtr/Fos: t(5) = 0.4049; p = 0.7023) and (e) and hypertonic saline experiments (n = 4 for 1 M saline, 3 normal saline; unpaired 2-tailed Student’s t-test; Fos/Oxtr: t(5) = 0.5084; p = 0.6328; Oxtr/Fos: t(5) = 1.319; p = 0.2444). b,d,f, Quantification of rostral OxtrPBN co-expression of Fos and Oxtr in (b) salt depletion (n = 4 salt returned, 4 salt depleted, 3 control; 1-way ANOVA; Fos/Oxtr: interaction F(2,7) = 2.008; p = 0.2046; Oxtr/Fos: interaction F(2,7) = 1.582; p = 0.2711), (d) fluid deprivation (n = 4 fluid returned, 3 fluid deprived; unpaired 2-tailed Student’s t-test; Fos/Oxtr: t(5) = 0.5977; p = 0.5761; Oxtr/Fos: t(5) = 0.3396; p = 0.7479) and (f) and hypertonic saline (n = 4 for 1 M saline, 3 normal saline; unpaired 2-tailed Student’s t-test; Fos/Oxtr: t(5) = 1.716; p = 0.1469; Oxtr/Fos: t(5) = 2.300; p = 0.0698). Data are expressed as mean ± s.e.m. See Supplementary Information for statistical analyses
