Fig. 2: OxtrPBN activation decreases food intake in dehydrated mice and suppresses water intake following 48-h salt depletion and thirst-inducing conditions.
From: Oxytocin-receptor-expressing neurons in the parabrachial nucleus regulate fluid intake
a, Acute OxtrPBN stimulation decreases food intake when mice are dehydrated (n = 6 hM3Dq, 7 mCherry; two-way RM ANOVA; interaction F(4,44) = 7.143, P = 0.0002). b–e, Acute OxtrPBN stimulation also decreases water, but not NaCl, consumption following 48-h salt depletion (n = 7/group; two-way RM ANOVA; NaCl: interaction F(8,96) = 1.408, P = 0.2028; water: interaction F(8,96) = 35.57, P < 0.0001) (b), 0.5 M saline ip injection (n = 7/group; two-way RM ANOVA; NaCl: interaction F(4,48) = 0.08955, P = 0.9853; water: interaction F(4,48) = 11.37, P < 0.0001) (c), 1 M mannitol ip injection (n = 7/group; two-way RM ANOVA; NaCl: interaction F(4,48) = 3.638, P = 0.0114; water: interaction F(4,48) = 24.56, P < 0.0001) (d) and 30% PEG sc injection (n = 6 hM3Dq, 7 mCherry; two-way RM ANOVA; NaCl: interaction F(4,44) = 0.4042, P = 0.8046; water: interaction F(4,44) = 11.54, P < 0.0001) (e). f, Following CNO administration, Fos was robustly expressed in OxtrPBN and adjacent neurons in hM3Dq-injected mice. Scale bar represents 100 µm. Data are expressed as mean ± s.e.m. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05. See Supplementary Table 2 for statistical analyses.
