Fig. 5: CGRPPBN neuron activation decreases fluid and salt intake. | Nature Neuroscience

Fig. 5: CGRPPBN neuron activation decreases fluid and salt intake.

From: Oxytocin-receptor-expressing neurons in the parabrachial nucleus regulate fluid intake

Fig. 5: CGRPPBN neuron activation decreases fluid and salt intake.The alt text for this image may have been generated using AI.

a, Minimal colocalization of Oxtr and CGRP in the PBN. Scale bar represents 200 µm (n = 3). b, Injection of AAV-DIO-hM3Dq:mCherry in CGRPPBN neurons. c–e Acute CGRPPBN activation with CNO produces a significant decrease in water consumption at baseline (n = 8 hM3Dq, 7 mCherry; three-way mixed design ANOVA; NaCl: interaction F(1.876,24.391) = 0.921, P = 0.406; water: interaction F(2.629,34.177) = 3.768, P = 0.023) (c) and after 24-h dehydration (n = 8 hM3Dq, 7 mCherry; two-way RM ANOVA; NaCl: interaction F(8,104) = 0.07589, P = 0.6395; water: interaction F(8,104) = 22.22, P < 0.0001) (d), and decreased NaCl and water consumption after 48-h salt depletion (n = 8 hM3Dq, 7 mCherry; two-way RM ANOVA; NaCl: interaction F(8,104) = 5.789, P < 0.0001; water: interaction F(8,104) = 14.01, P < 0.0001) (e). f, Following CNO, Fos is robustly expressed in external lateral CGRPPBN neurons and in dorsolateral PBN in hM3Dq-injected mice. Scale bar represents 200 µm. Data expressed as mean ± s.e.m. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05. See Supplementary Table 2 for statistical analyses.

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