Supplementary Figure 7: Silencing of visual cortex using muscimol applied to the intact dura | Nature Neuroscience

Supplementary Figure 7: Silencing of visual cortex using muscimol applied to the intact dura

From: Lateral geniculate neurons projecting to primary visual cortex show ocular dominance plasticity in adult mice

Supplementary Figure 7

a/c) Activity maps (maximum projections over 13 min of recording) of the same field of view during visual stimulation before and after muscimol application in layer 2/3 (a) and layer 5/6 cells (c) in visual cortex of GCaMP6 animals (CamKIIa-tTa;Tg(tetO-GCaMP6s)2Niell). Overlay: Contra- (blue) and ipsilalateral (red) population responses (mean ± SEM) of layer 2/3 cells as well as binocular (gray) population response of layer 5/6 cells in V1 in response to preferred drifting grating stimulation (white bars: time of stimulus presentation). Note lack of responses ~2h after topical application of muscimol. b) Median change in Ca2+ signal in response to visual stimulation of the ipsilateral or contralateral eye, respectively, in layer 2/3 cells. On each box-plot here and below, the central mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The whiskers extend to the most extreme data points not considered outliers (1.5 times the interquartile range, outliers not shown; 2 mice, n = 62 cells; ipsilateral eye response, Z(61) = 6.7, P < 10-10; contralateral eye response, Z(61) = 6.7, P < 10-10; Wilcoxon signed-rank tests. d) Median response amplitudes to binocular stimulation before and after muscimol application recorded at a depth of 590-650 µm below dura (putatively deep layer 5, upper layer 6; 3 mice; pre-muscimol, n = 139 cells, post-muscimol, n = 120 cells; Z(1, 257) = 12.6, P = 0, Wilcoxon rank sum test). e) V1 activation, as measured with intrinsic optical imaging, evoked by stimulation of the contralateral (blue) or ipsilateral (red) eye before and after muscimol application (n = 2 mice, mean ± SEM). f) Median response amplitude to visual stimulation after application of the GABA-A agonist muscimol together with the GABA-B antagonist SCH50911 of thalamic boutons in layer 1 of V1 in wildtype mice (n = 251 boutons in 3 animals, ipsilateral eye responses, Z(250) = 4.2, P = 0.33; contralateral eye responses, Z(250) = 1.0, P < 10-4; Wilcoxon signed-rank)

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