Supplementary Figure 5: OD plasticity and recovery from OD plasticity in thalamic afferents
a) Schematic of stimulus presentation for intrinsic optical imaging. Visual stimuli were presented at two different locations on a screen centered on the binocular visual field of the mouse. b) Color-coded maps (see a) of cortical responses to stimulation of the ipsilateral (top panel) or contralateral (bottom panel) eye (scale bar: 0.5 mm), overlaid on an image of the cortical surface blood vessel pattern. c) ODI values based on intrinsic optical imaging of cortical responses for normal adult mice (mean ± SEM: 0.27 ± 0.04, n = 12), after 6-8 days of MD (-0.06 ± 0.07, n = 12), and after 1-2 weeks of recovery (0.15 ± 0.05, n = 6 animals, χ2(2) = 22.3, P < 10-5; Kruskal-Wallis test, Bonferroni corrected, baseline vs. MD, P < 10-5; baseline vs. recovery, P = 0.22; MD vs. recovery, P = 0.41). d) Example time course of dLGN single-bouton ODI (gray lines) from one animal over baseline, contralateral eye MD and recovery (black line: mean ODI). Conventions for sessions quantified are indicated on top (mean pre-MD, pre-MD, post-MD, and recovery). e) OD Shift magnitude of boutons with receptive field size < 200deg2. Single-bouton ODI distribution during baseline (mean of three pre-MD sessions, ODI = 0.35 ± 0.67, standard deviation, SD) and after 6-8 days of MD (post-MD: ODI = 0.20 ± 0.58, SD, n = 78 boutons, 5 animals, Z(77) = 2.6, P = 0.01, Wilcoxon signed-rank test, compare Fig. 2c). Lines connect individual, continuously responsive boutons; line color indicates shift significance in units of standard deviations over pre-MD baseline fluctuations. Colored ODI histogram bins indicate class definitions for contralateral (blue), binocular (white) and ipsilateral (red) boutons (see Fig 2c). ( f) Eye-specific fluorescence changes in response to drifting grating stimulation (8 directions) in 1507 dLGN boutons in 12 animals before and after MD. All responses are sorted for preferred direction (horizontally) and contralateral or ipsilateral eye response magnitude (vertically) in each session (as indicated by the black wedges; blue and red bars: time of stimulus presentation). Note the decrease in contra- and increase in ipsilateral eye responsiveness. g) Mean ODI ( ± SEM) of boutons (n = 132 boutons from 5 mice, responsive during baseline, MD and recovery) after MD and after at least one week of binocular vision (χ2(2) = 59.3, P < 10-12; baseline vs. MD, P < 10-7; MD vs. recovery, P < 10-11; baseline vs. recovery, P = 0.47; Friedman test, Bonferroni corrected). Lines here and in (h,i) connect individual, continuously responsive boutons. h) Mean response amplitude ( ± SEM) to ipsilateral eye stimulation after MD and at least one week of binocular vision (χ2(2) = 59.3, P < 10-10; baseline vs. MD, P < 10-5; MD vs. recovery, P < 10-10; baseline vs. recovery, P = 0.25; Friedman test, Bonferroni corrected). i) Mean response amplitude ( ± SEM) to contralateral eye stimulation after MD and at least one week of binocular vision (χ2(2) = 11.2, P = 0.004; baseline vs. MD, P = 0.003; MD vs. recovery, P = 0.07; baseline vs. recovery, P = 0.97; Friedman test, Bonferroni corrected)
