Supplementary Figure 1: Generation of conditional Mettl14-knockout mice

(a) Schematic diagram showing strategy used to create conditional Mettl14 knockout. (b-d) Representative genotyping PCR results using different primer sets for the targeted Mettl14 allele (b), the floxed Mettl14 allele (c), or the exon 2-deleted Mettl14 allele (d). Similar results were obtained from three independent experiments. (e) Representative images of wildtype and Mettl14 null embryos at E7.5. Similar results were obtained from three independent experiments. (f) Representative PCR results for sex determination. Similar results were obtained from three independent experiments. (g) Representative images of pups at P0. Scale bar: 5 mM. (h) Quantification of pup body weight at P0, one-way ANOVA (WT: n = 16, KO: n = 7, and Het: n = 8 P0 pups; P = 0.1719, F (2, 28) = 1.877) followed by Bonferroni's post hoc test (WT vs. KO, P = 0.4198, 95% C.I. = −0.09762 to 0.3285, WT vs. Het, P = 0.6836, 95% C.I. = −0.2867 to 0.1205). (i) Survival curve of newborn mice with or without brain-specific Mettl14 deletion (Mettl14 f/f: n = 12, Mettl14 f/+: n = 10, Mettl14 f/+;nes-cre: n = 12, Mettl14 f/f;nes-cre: n = 14 animals). Graphs represent mean ± SD. Dots represent data from individual data points. ns = non-significant.