Supplementary Figure 5: HSD increases mRNA levels of factors required for Th17 polarization.
From: Dietary salt promotes neurovascular and cognitive dysfunction through a gut-initiated TH17 response
A: HSD induces upregulation of IL-22, IL-23R, iNOS, SAA1-3 in distal small intestine of mice fed a HSD. IL-22: * p = 0.0073 vs ND; ND/HSD n = 7/8; IL-23R: * p = 0.0057 vs ND; ND/HSD n = 8/7; iNOS: * p = 0.0005 vs ND; ND/HSD n = 8/9; SAA1: * p = 0.0007 vs ND; ND/HSD n = 8/9; SAA2: * p = 0.0005 vs ND; ND/HSD n = 8/9; SAA3: * p < 0.0001 vs ND; ND/HSD n = 8/9 mice/group (unpaired t-test, two-tailed). B: HSD does not increase IL-17A mRNA levels in the colon. mRNA levels are normalized to IL-17A mRNA levels in the distal small intestine of mice fed a ND. Diet: p = 0.1461, ND/HSD n = 3/4 mice/group (Two-way ANOVA and Tukey's test). C: Plasma levels of TNF-α and IL-6 are not increased after HSD. TNF-α: p = 0.6581 vs ND; IL-6: p = 0.8592 vs ND; n = 12 mice/group (unpaired t-test, two-tailed). n.d: not detectable. Data are expressed as mean ± SEM.
