Supplementary Figure 10: Effect of IL-17 and TNF-α administration on mRNA levels of proinflammatory mediators in brain endothelial cells (bEnd.3).
From: Dietary salt promotes neurovascular and cognitive dysfunction through a gut-initiated TH17 response
A: IL-17 (10 ng/mL) does not induce upregulation of cytokines, adhesion molecules and other pro-inflammatory molecules in cultures of bEnd.3 cells. p > 0.05 vs 0; n = 4 independent experiments/group (One-way ANOVA and Tukey’s test). B: TNF-α (10 ng/mL) induces upregulation of cytokines and adhesion molecules in cultures of bEnd.3 cells. ICAM1: * p = 0.0124 vs 0; VCAM1: * p < 0.0001 vs 0; CXCL1: * p = 0.0024; CXCL2: * p = 0.0035 vs 0; CXCL5: * p = 0.0372 vs 0; MCP1: * p < 0.0001 vs 0; n = 3 independent experiments/group (One-way ANOVA and Tukey’s test). Data are expressed as mean ± SEM.
