Supplementary Figure 7: Representative examples of c-Fos immunohistochemistry and regions of interest.
From: Insular cortex mediates approach and avoidance responses to social affective stimuli
Coronal diagrams on left correspond to regions of interest indicated within the representative digital photomicrographs on the right. Images were acquired and analyzed with equal exposure and analysis was conducted on images without adjustments to brightness or contrast. The representative images have been adjusted to facilitate presentation. This experiment was replicated 11 times per group. List of abbreviations is provided in Supplementary Figure 8. Scale bars = 200 μm. Brain Atlas illustrations were reproduced with permission as previously published in The Brain Atlas in Stereotaxic Coordinates, 4th Edition, Paxinos, G. & Watson, C. Pages 296, 303, 306–317 & 332. Copyright Elsevier (1998).
