Supplementary Figure 6: dCas9-based Syt1 enhancement in primary neurons.
From: CRISPR interference-based specific and efficient gene inactivation in the brain
(a) Schematic diagram of dCas9-based synergistic activation mediator (dCas9-SAM) system for Syt1 activation. Compass arrowheads indicate the sgRNA targeting sites. (b,c) Immunoblot analysis (b) and quantification (c) of Syt1 protein level in neurons expressing the dCas9-SAM system (n = 3 for all groups). In b, blots were cropped; full-length blots are presented in Supplementary Figure 9. (d) qRT-PCR analysis of Syt1 enhancement by the dCas9-SAM system (n = 3 for all groups). Data were obtained from neurons from three independent cultures with similar results and shown as mean ± s.e.m. Statistical significance was assessed by Unpaired Student’s t-test. *P < 0.05. For detailed numbers and statistical analysis, see Supplementary Table 3.
