Supplementary Figure 4: Expression and function of Syt2 and Syt9 in GABAergic hippocampal neurons.

(a) The mRNA expression of Syt1/2/9 in the pVGAT::dCas9-KRAB⁺ GABAergic neurons and pVGAT::dCas9-KRAB^- glutamatergic neurons (n = 3 for all groups). Data were obtained from neurons from three independent cultures with similar results and shown as mean ± s.e.m. Statistical significance was assessed by Unpaired Student’s t-test. **P = 0.001. (b, c) Representative traces (b) and average peak amplitudes (c) of AP-evoked IPSCs showing functions of Syt2 and Syt9 in the pVGAT::dCas9-KRAB⁺ GABAergic neurons (n = 35, 28, 13, 32). Data were obtained from neurons from three independent cultures with similar results, n represents the number cells analyzed. Quantification is represented as a box-and-whisker plot with upper and lower whiskers representing the maximum and minimum values, respectively; the boxes represent 2.5%, median and 97.5% quartiles. Statistical significance was assessed by Unpaired Student’s t-test; **P < 0.001. For detailed numbers and statistical analysis, see Supplementary Table 3.