Supplementary Figure 4: Dopamine signaling regulated optogenetic LTP in DMS slices.
From: Bidirectional and long-lasting control of alcohol-seeking behavior by corticostriatal LTP and LTD
(a) Optogenetic induction of LTP in the presence of the dopamine D1R antagonist, SCH 23390 (10 µM, left), and agonist, SKF 38393 (20 µM, right) in DMS slices of rats. AAV-C1V1-eYFP was infused into the DMS. The grey lines are the control LTP from Fig. 1d for reference. Left, suppression of LTP by SCH 23390 (108.65 ± 1.95% of baseline, t(5) = -4.44, P = 0.0068; n = 6 slices, 6 rats). Right, enhancement of LTP by SKF 38393 (128.84 ± 2.69% of baseline, t(7) = -10.70, P < 0.0001; n = 8 slices, 5 rats). Sulpiride was bath-applied to block LTD and favor LTP induction. (b) Comparison of the magnitudes of LTP in control and in the presence of SCH 23390 and of SKF 38393 in rats. The grey dots are from Fig. 1d for comparison (SCH 23390 vs Control: t(14) = 2.33, *P = 0.035; SKF 38393 vs Control: t(16) = -2.56, *P = 0.021). n = 10 slices from 6 rats (Control), 6 slices from 6 rats (SCH 23390), and 8 slices from 5 rats (SKF 38393). Two-sided paired t test for a; two-sided unpaired t test for b. Data are presented as mean ± s.e.m.
