Supplementary Figure 7: In vivo delivery of the optogenetic LTP-inducing protocol facilitated alcohol- but not sucrose-seeking behavior.
From: Bidirectional and long-lasting control of alcohol-seeking behavior by corticostriatal LTP and LTD
(a) In vivo optogenetic LTP induction persistently increased alcohol deliveries. F(5,54) = 3.83, P = 0.0048, *P < 0.05 vs. baseline (BL). n = 14 rats. (b,c) In vivo LTP induction increased blood alcohol concentration (BAC) at 30 min (b) and 2 days (c) after induction. 30 min: t(5) = -4.25, P = 0.0081, n = 6 rats; 2 days: t(5) = -3.36, P = 0.02, n = 6 rats. *P < 0.05 and **P < 0.01 vs. baseline (BL). (d) In vivo oHFS stimulation alone did not persistently alter lever presses for alcohol (left; F(4,17) = 4.38, P = 0.013, n = 6 rats), alcohol deliveries (middle; F(4,17) = 4.67, P = 0.01, n = 6 rats), or alcohol intake (right; F(4,17) = 0.59, P = 0.67, n = 6 rats). *P < 0.05. (e) In vivo oPSD stimulation alone did not alter lever presses for alcohol (left; F(4,26) = 0.63, P = 0.65, n = 8 rats), alcohol deliveries (middle; F(4,26) = 0.41, P = 0.80, n = 8 rats), or alcohol intake (right; F(4,26) = 0.6, P = 0.66, n = 8 rats). (f) In vivo LTP induction in the presence of a D1R antagonist, SCH 23390 (SCH, 0.01 mg/kg), failed to alter lever presses (left; F(4,40) = 0.66, P = 0.63), alcohol deliveries (middle; F(4,39) = 1.26, P = 0.30), or alcohol intake (right; F(4,39) = 0.58, P = 0.68). n = 12 rats. (g) In vivo LTP induction in the presence of MK801 failed to alter lever presses (left; t(4) = -2.09, P = 0.11), alcohol deliveries (middle; t(4) = -2.07, P = 0.11), or alcohol intake (right; t(4) = -0.60, P = 0.58). n = 5 rats. (h) Systematic administration of SCH 23309 did not alter lever presses (left; t(13) = 0.16, P = 0.87), alcohol deliveries (middle; t(13) = 0.03, P = 0.98), or alcohol intake (right; t(13) = -0.85, P = 0.41), as compared to baseline (BL). n = 14 rats. (i) In vivo LTP induction did not alter sucrose deliveries (left) or intake (right). Deliveries: F(4,19) = 0.84, P = 0.51; intake: F(4,18) = 1.94, P = 0.15, n = 6 rats. One-way RM ANOVA for a,d-f,i; two-sided paired t test for b, c, g, and h. Data are presented as mean ± s.e.m.
