Supplementary Figure 11: In vivo delivery of the LTP- and LTD-inducing protocols to mPFC inputs onto D1-MSNs distinctly modulated alcohol seeking-behavior.
From: Bidirectional and long-lasting control of alcohol-seeking behavior by corticostriatal LTP and LTD
(a) In vivo delivery of the LTP-inducing protocol (oHFS+oPSD) to the mPFC input onto DMS D1-MSNs induced a persistent increase in alcohol-deliveries. *P < 0.05 vs. baseline (BL). F(4,23) = 4.77, P = 0.0060; n = 8 rats. (b, c) In vivo oPSD of D1-MSNs alone did not alter alcohol deliveries (b; F(4,18) = 0.32, P = 0.86) or intake (c; F(4,18) = 0.76, P = 0.56; n = 6 rats). (d) In vivo delivery of LTD-inducing protocol (oHFS+oPSD+MK801+raclopride) to the mPFC input onto DMS D1-MSNs produced a long-lasting attenuation in alcohol deliveries. *P < 0.05 vs. baseline (BL). F(4,23) = 4.99, P = 0.0048; n = 7 rats. (e,f) In vivo delivery of the LTD-inducing protocol in the presence of AM251 (oHFS+oPSD+MK801+raclopride+AM251) failed to alter alcohol deliveries (e; F(4,20) = 0.24, P = 0.91) or intake (f; F(4,19) = 1.67, P = 0.20; n = 7 rats). One-way RM ANOVA for all figures. Data are presented as mean ± s.e.m.
