Supplementary Figure 12: In vivo delivery of the LTP- or LTD-inducing protocol did not alter inactive lever presses.
From: Bidirectional and long-lasting control of alcohol-seeking behavior by corticostriatal LTP and LTD
(a-b) In vivo delivery of the optogenetic LTP-inducing protocol did not change inactive lever presses for alcohol (a, F(5,54) = 0.41, P = 0.84; n = 14 rats; related to Fig. 3b) or for sucrose (b, F(4,18) = 0.31, P = 0.87; n = 6 rats; related to Fig. 3c). (c) In vivo delivery of the optogenetic LTD-inducing protocol did not change inactive lever presses for alcohol. F(5,30) = 1.05, P = 0.41; n = 9 rats; related to Fig. 4b. (d) In vivo delivery of the optogenetic LTD-inducing protocol in the presence of AM251 did not change inactive lever presses for alcohol. F(4,30) = 1.33, P = 0.28; n = 9 rats; related to Fig. 4c. (e-f) In vivo delivery of the LTP-inducing protocol (oHFS+oPSD) (e, F(4,25) = 0.53, P = 0.72; n = 8 rats; related to Fig. 7c) or oPSD (f, F(4,16) = 1.44, P = 0.27; n = 6 rats; related to Fig. 7d) to the mPFC input onto DMS D1-MSNs did not affect inactive lever presses for alcohol. (g) In vivo delivery of the LTD-inducing protocol to the mPFC input onto DMS D1-MSNs did not alter inactive lever presses for alcohol. F(4,18) = 1.59, P = 0.22; n = 6 rats; related to Fig. 7e. (h) In vivo delivery of the LTD-inducing protocol to the mPFC input onto DMS D1-MSNs in the presence of AM251 decreased inactive lever presses for alcohol only at 30 min, but not on day 2, 4, or 7. *P < 0.05 versus baseline (BL). F(4,23) = 5.04, P = 0.0046; n = 7 rats; related to Fig. 7f. (i-j) In vivo delivery of oHFS (i, F(4,17) = 1.86, P = 0.16; n = 6 rats; related to Supplementary Figure 7d) or oPSD (j, F(4,25) = 1.39, P = 0.26; n = 8 rats; related to Supplementary Figure 7e) did not cause any change on inactive lever presses for alcohol. (k-l) In vivo delivery of the LTP-inducing protocol in the presence of SCH 23390 (k, F(4,43) = 0.57, P = 0.69; n = 12 rats; related to Supplementary Figure 7f) or MK801 (l, t(4) = 0, P = 1; n = 5 rats; related to Supplementary Figure 7g) did not change the inactive lever presses for alcohol. (m) Systematic administration of SCH 23390 did not alter inactive lever presses at 30 min (t(13) = 0.16, P = 0.87; n = 13 rats; related to Supplementary Figure 7h). (n) In vivo delivery of oHFS with administration of MK801 and raclopride did not change inactive lever presses for alcohol. F(4,28) = 1.17, P = 0.34; n = 9 rats; related to Supplementary Figure 10b. (o) Systematic administration of a cocktail of MK801 and raclopride did not affect the inactive lever presses for alcohol. F(4,24) = 0.96, P = 0.45; n = 8 rats; related to Supplementary Figure 10e. (p) Systematic administration of AM251 (0.3 mg/kg) did not alter inactive lever presses for alcohol. F(2,18) = 2.04, P = 0.16; n = 10 rats; related to Supplementary Figure 10j. One-way RM ANOVA for Figures a-k and n-p; two-sided paired t test for Figures l and m. Data are presented as mean ± s.e.m.
